Tutorials

Solid-State NMR – Magic Angle Spinning (MAS) and organic solids

• Instructions for MAS Sample Packing
• Video for packing Bruker rotors (Bruker narration is loud)
• Video for removing and inserting Phoenix/Revolution NMR caps (no narration)
• Tuning and Matching MAS Probe
• Adjusting the Magic-Angle
• Adamantane – ¹H/¹³C Pulse Calibration/Shimming/Chemical Shift Reference
• Typical Organic Solids – ¹³C Cross Polarization Setup, ¹H T₁, CP Match and Build-up

Magic Angle Spinning (MAS) and DNP

• Instructions for DNP sample preparation – packing and data collection MW on/off, DNP build-up

Metabolomics – Estimate fluxes using ¹³C and ¹H NMR signals for samples derived from cell culture

• Study Design
• Cell Culture
• Metabolite Extraction
• NMR Sample Preparation, Spectroscopy and Data Processing
• Metabolic Modelling

Metabolomics Tutorial adapted from: Ragavan, M., & Merritt, M. E. (2019). Nuclear Magnetic Resonance Measurement of Metabolic Flux Using ¹³C and ¹H Signals. In Metabolomics (pp. 29-40). Humana, New York, NY.